A Walk Through Nature Part III: Catalytic RNA Unworthy Of An ‘Origins’ Discussion

I would like to know how an RNA world is even plausible if synthesizing the different chemical constituents (nitrogenous bases, carbon sugars, phosphate backbone, etc.) occurs under different respective conditions. Needless to say, last year's big excitement over the the RNA world was a bit misplaced... http://www.evolutionnews.org/2009/02/origin_of_life_researchers_int.htmlF2XL

March 6, 2010

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For an analysis of the Catalytic RNA in english, instead of spanish, there is this article: Origin of Life: Claiming Something for Almost Nothing: http://www.creationsafaris.com/crev201003.htm#20100302a Excerpt of their lab work: Here’s how the paper described their work in the lab to get this meager result:

RNA was synthesized by Dharmacon. GUGGC = 5’-GUGGC-30 ; GCCU – 5’P-GCCU-3’ ; 5’OH-GCCU = 5’-GCCU-3’ ; GCCU20dU = 5’-GCC-2’-dU; GCC = 5’-GCC-3’ ; dGdCdCrU = 5’-dGdCdCU-3’ . RNA GCC3’dU was prepared by first synthesizing 5’-O-(4,4’- Dimethoxytrityl)3’-deoxyuridine as follows: 3’-deoxyuridine (MP Biomedicals; 991 mg, 0.434 mmol) was dissolved in 5 mL anhydrous pyridine and pyridine was then removed under vacuum while stirring. Solid was then redissolved in 2 mL pyridine. Dimethoxytrityl chloride (170 mg, 0.499 mmol) was dissolved in 12 mL pyridine and slowly added to 3’-deoxyuridine solution. Solution was stirred at room temperature for 4 h. All solutions were sequestered from exposure to air throughout. Reaction was then quenched by addition of 5 mL methanol, and solvent was removed by rotary evaporation. Remaining solvent evaporated overnight in a vacuum chamber. Product was then dissolved in 1 mL acetonitrile and purified through a silica column (acetonitrile elution). Final product fractions (confirmed through TLC, 1.1 hexane:acetonitrile) were pooled and rotary evaporated. Yield was 71%. Dimethoxytrityl-protected 30dU was then sent to Dharmacon for immobilization of 30-dU on glass and synthesis of 5’-GCC-3’-dU. PheAMP, PheUMP, and MetAMP were synthesized by the method of Berg (25) with modifications and purification as described in ref. 6. Yield was as follows: PheAMP 85%, PheUMP 67%, and MetAMP 36%. Even more purification and isolation steps under controlled conditions, using multiple solvents at various temperatures, were needed to prevent cross-reactions.

This understatement followed:

It is doubtful such complex lab procedures have analogues in nature.

Or as Dr. Meyer has stated previously: Biological Information: The Puzzle of Life that Darwinism Hasn’t Solved - Stephen C. Meyer Thus, as my book Signature in the Cell shows, Joyce’s experiments not only demonstrate that self-replication itself depends upon information-rich molecules, but they also confirm that intelligent design is the only known means by which information arises. http://www.evolutionnews.org//2009/06/biological_information_the_puz.html and here: Stephen Meyer Responds to Fletcher in Times Literary Supplement - Jan. 2010 Excerpt: everything we know about RNA catalysts, including those with partial self-copying capacity, shows that the function of these molecules depends upon the precise arrangement of their information-carrying constituents (i.e., their nucleotide bases). Functional RNA catalysts arise only once RNA bases are specifically-arranged into information-rich sequences—that is, function arises after, not before, the information problem has been solved. http://www.evolutionnews.org/2010/01/stephen_meyer_responds_to_flet.htmlbornagain77

March 5, 2010

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